Preparation of Single-Stranded Phagemid DNA Without Chromosomal DNA Contamination
نویسندگان
چکیده
منابع مشابه
Preparation of single-stranded phagemid DNA without chromosomal DNA contamination.
During the culture of phagemidcontaining bacteria infected with M13KO7 helper phage, many host strains such as JM109 and XL1-Blue release their chromosomal DNA into the medium because of lysis of bacteria. This results in chromosomal DNA contamination of the preparation of single-stranded DNA (ssDNA) (2,5). A certain strain of E. coli, DH11S, has been engineered to overcome this problem (2). Ho...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1996
ISSN: 0736-6205,1940-9818
DOI: 10.2144/96211bm10